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KMID : 0545120070170101733
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 10 p.1733 ~ p.1737
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Investigation of β-Lactamase-producing Multidrug-resistant Pseudomonas aeruginosa Isolated from Non-Tertiary Care Hospitals in Korea
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Sohn Eui-Suk
Yoo Jung-Sik
Lee Jeom-Kyu
Lee Kyeong-Min
Chung Gyung-Tae
Shin Eun-Shim
Han Sun-Young
Lee Sang-Hee
Kim Joon
Lee Yeong-Seon
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Abstract
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A total of 2,280 nonduplicate clinical isolates of Pseudomonas aeruginosa, obtained nationwide from Korean non-tertiary care hospitals from 2002 to 2005, were identified and their susceptibilities to aminoglycosides, antipseudomonal penicillins, carbapenems, cephalosporins, monobactams, and quinolones were studied, together with their production of β- lactamases. Using disk diffusion and minimum inhibitory concentration tests, it was found that 2.9% of isolates were multidrug-resistant (MDR) P. aeruginosa. An EDTA-disk synergy test, PCR amplification with specifically designed primers, and direct sequencing of the PCR products showed that the blaOXA-10, blaVIM-2, blaOXA-2, blaOXA-17, blaPER-1, blaSHV-12, and blaIMP-1 genes were carried by 34.3%, 26.9%, 3.0%, 3.0%, 1.5%, 1.5%, and 1.5% of 67 MDR P. aeruginosa isolates, respectively. The prevalence of MDR P. aeruginosa was three-fold higher, compared with that from the United States. More than two types of β-lactamase genes were carried by 10.4% of isolates. The most prevalent β-lactamase genes were blaVIM-2 and blaOXA-10. This study is the first description of MDR P. aeruginosa from non-tertiary care hospitals in Korea and the coexistence of the blaOXA-10 gene with blaVIM-2, blaIMP-1, or blaPER-1 in these clinical isolates.
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KEYWORD
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Multidrug resistance, Pseudomonas aeruginosa, VIM-2, OXA-10, PER-1
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